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高含量成像分析研究。
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JoVE Journal Chemistry
A Rhodopsin Transport Assay by High-Content Imaging Analysis
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高含量成像分析研究。

DOI:
10.3791/58703-v

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12:11 min

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January 16, 2019

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, ,
1Department of Ophthalmology,University of Pittsburgh, 2McGowan Institute for Regenerative Medicine,University of Pittsburgh

Chapters

  • 00:04Title
  • 01:19Treating the Cells with Compounds
  • 02:19Immunostaining without Membrane Permeabilization to Stain Rhodopsin Protein on the Cell Surface
  • 04:43Imaging
  • 07:12Image Analysis
  • 09:12Results: Characterization of Rhodopsin Transport
  • 10:50Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们描述了一种高含量的成像方法来量化与视网膜色素变性相关的视紫红质突变体的转运。采用多波长评分分析法对细胞表面或整个细胞的视紫红质蛋白进行了定量。

Tags

Rhodopsin TransportRetinitis PigmentosaHigh-content Imaging AnalysisMembrane Protein Localization384-well Plate AssayImmunostainingDrug ScreeningZ-factor9-cis-retinal

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