Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double <em>In Vitro</em> Transcription with Absolute Counts Sequencing (DIVA-Seq)

Anirban Paul; Z. Josh Huang

doi:10.3791/58690

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Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing (DIVA-Seq)

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Neuroscience

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JoVE Journal Neuroscience

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing (DIVA-Seq)

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing (DIVA-Seq)

9,282 Views

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07:49 min

•

October 26, 2018

DOI:

10.3791/58690-v

DOI:

10.3791/58690-v

•

07:49 min

October 26, 2018

•

1 Views

Anirban Paul¹, Z. Josh Huang¹

¹Cold Spring Harbor Laboratory

Summary

This protocol describes the manual sorting procedure to isolate single fluorescently labeled neurons followed by in vitro transcription-based mRNA amplification for high-depth single-cell RNA sequencing.

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Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing (DIVA-Seq)

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Summary

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