Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double <em>In Vitro</em> Transcription with Absolute Counts Sequencing (DIVA-Seq)

Anirban Paul; Z. Josh Huang

doi:10.3791/58690

/

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing (DIVA-Seq)

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JoVE Journal Nörobilim

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing (DIVA-Seq)

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing (DIVA-Seq)

DOI:

10.3791/58690-v

•

07:49 min

•

October 26, 2018

•

Anirban Paul, Z. Josh Huang

¹Cold Spring Harbor Laboratory

Bölümler

00:04Başlık
00:43Preparation for Dissection and Neuron Sorting
02:09Brain Sectioning and Cell Dissociation
04:15Manual Sorting of Fluorescently Labeled Mouse Neurons
05:59Results: Microdissection of GABAergic Neurons
07:24Conclusion

Özet

Otomatik Çeviri

This protocol describes the manual sorting procedure to isolate single fluorescently labeled neurons followed by in vitro transcription-based mRNA amplification for high-depth single-cell RNA sequencing.

Etiketler

Single-cell RNA Sequencing Fluorescently Labeled Neurons Manual Sorting Double In Vitro Transcription DIVA-Seq Neuron Isolation Subpopulation FACS Sorting Glass Microcapillaries Artificial Cerebrospinal Fluid Protease FBS Pasteur Pipettes Brain Dissection Vibratome Labeled Cells Activity Blockers

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