このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Chromatin Immunoprecipitation of Murine Brown Adipose Tissue
7,895 Views
•
07:50 min
•
November 21, 2018
DOI:
概要
Automatically generated
Here we describe a protocol for efficient chromatin immunoprecipitation (ChIP) followed by high-throughput DNA sequencing (ChIP-seq) of brown adipose tissue (BAT) isolated from a mouse. This protocol is suitable for both mapping histone modifications and investigating genome-wide localization of non-histone proteins of interest in vivo.
Read Article
Cite this Article
Cardamone, M. D., Orofino, J., Labadorf, A., Perissi, V. Chromatin Immunoprecipitation of Murine Brown Adipose Tissue. J. Vis. Exp. (141), e58682, doi:10.3791/58682 (2018).
Copy