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Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies
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Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies

Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies

DOI:
10.3791/58650-v

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08:41 min

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November 08, 2018

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, , , ,
1USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology, 2CMAVE Detachment,Navy Entomology Center of Excellence, 3Lovelace Respiratory Research Institute

Chapters

  • 00:04Title
  • 00:53Insect Preparation
  • 01:21Insect Injection
  • 03:57Aedes aegypti Mortality and Oviposition Bioassay
  • 05:22Musca domestica Mortality and Oviposition Bioassay
  • 07:08Results: Microinjection with dsRNA Leads to Reduced Specific Transcript Expression and Clutch Size in Aedes aegypti and Musca domestica
  • 08:10Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a microinjection methodology that we have standardized and used for several years to deliver specific quantities of nucleic acids directly to the hemolymph of mosquitoes and house flies. This protocol results in minimal injection mortality and allows dose correlated measurements of fecundity.

Tags

Keywords: DsRNAMicroinjectionOvipositionBioassayMosquitoesHouse FliesBiopesticidesGene ExpressionFecundityAedes AegyptiMusca DomesticaForcepsMicroscope SlideChill TableGlass CapillariesRhodamine BInjection SolutionMesocarape SternumCuticle

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