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Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies
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Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies

Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies

English

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8,528 Views

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08:41 min

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November 08, 2018

DOI:

10.3791/58650-v

DOI:
10.3791/58650-v

•

08:41 min
November 08, 2018

•

8 Views
, , , ,
1USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology, 2CMAVE Detachment,Navy Entomology Center of Excellence, 3Lovelace Respiratory Research Institute

Summary

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This protocol describes a microinjection methodology that we have standardized and used for several years to deliver specific quantities of nucleic acids directly to the hemolymph of mosquitoes and house flies. This protocol results in minimal injection mortality and allows dose correlated measurements of fecundity.

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Sanscrainte, N. D., Waits, C. M., Geden, C. J., Estep, A. S., Becnel, J. J. Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies. J. Vis. Exp. (141), e58650, doi:10.3791/58650 (2018).

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