ジャーナル
/
環境学
/
Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies
JoVE Journal
環境学
This content is Free Access.
JoVE Journal 環境学
Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies

Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies

DOI:
10.3791/58650-v

•

08:41 min

•

November 08, 2018

•

, , , ,
1USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology, 2CMAVE Detachment,Navy Entomology Center of Excellence, 3Lovelace Respiratory Research Institute

章

  • 00:04標題
  • 00:53Insect Preparation
  • 01:21Insect Injection
  • 03:57Aedes aegypti Mortality and Oviposition Bioassay
  • 05:22Musca domestica Mortality and Oviposition Bioassay
  • 07:08Results: Microinjection with dsRNA Leads to Reduced Specific Transcript Expression and Clutch Size in Aedes aegypti and Musca domestica
  • 08:10Conclusion

概要

自動翻訳

自動翻訳

This protocol describes a microinjection methodology that we have standardized and used for several years to deliver specific quantities of nucleic acids directly to the hemolymph of mosquitoes and house flies. This protocol results in minimal injection mortality and allows dose correlated measurements of fecundity.

タグ

DsRNAMicroinjectionOvipositionBioassayMosquitoesHouse FliesBiopesticidesGene ExpressionFecundityAedes AegyptiMusca DomesticaForcepsMicroscope SlideChill TableGlass CapillariesRhodamine BInjection SolutionMesocarape SternumCuticle

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Optimize Flue Gas Settings to Promote Microalgae Growth in Photobioreactors via Computer Simulations

Detection of Foodborne Bacterial Pathogens from Individual Filth Flies

A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms

A Small Volume Bioassay to Assess Bacterial/Phytoplankton Co-culture Using WATER-Pulse-Amplitude-Modulated (WATER-PAM) Fluorometry

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

Visualizing Efficacy of Pesticides Against Disease Vector Mosquitoes in the Field

Colletotrichum fioriniae Development in Water and Chloroform-based Blueberry and Cranberry Floral Extracts

Leaf Area Index Estimation Using Three Distinct Methods in Pure Deciduous Stands

Read Article