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En fluorescens svingninger spektroskopi analysen av Protein-Protein interaksjoner i celle-celle kontakter
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
DOI:

08:43 min

December 01, 2018

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Chapters

  • 00:04Title
  • 00:49Sample Preparation: Cell-Cell Mixing Assay
  • 02:37Scanning Fluorescence Cross-Correlation Spectroscopy (sFCCS): Acquisition
  • 04:07Scanning Fluorescence Cross-Correlation Spectroscopy (sFCCS): Data Analysis
  • 06:03Results: Investigation of Protein-Protein Interactions at Cell-Cell Contacts by sFCCS
  • 08:09Conclusion

Summary

Automatic Translation

Denne protokollen beskriver en fluorescens svingninger spektroskopi tilnærming for å undersøke interaksjon mellom proteiner formidling celle-celle interaksjoner, dvs proteiner lokalisert i cellen veikryss, direkte i levende celler. Vi gir detaljerte veiledninger på instrumentet kalibrering, datainnsamling og analyse, inkludert rettelser til mulig gjenstanden kilder.

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