This content is Free Access.
1Interdisciplinary Stem Cell Institute,University of Miami Leonard M. Miller School of Medicine, 2Department of Medicine, Division of Cardiology,University of Miami Leonard M. Miller School of Medicine, 3Department of Molecular and Cellular Pharmacology,University of Miami Leonard M. Miller School of Medicine, 4Dr. John T. Macdonald Foundation Department of Human Genetics,University of Miami Leonard M. Miller School of Medicine, 5John P. Hussman Institute for Human Genomics,University of Miami Leonard M. Miller School of Medicine, 6Department of Medicine, Division of Endocrinology, Metabolism and Endocrinology and the Diabetes Research Institute,University of Miami Leonard M. Miller School of Medicine, 7Vascular Biology Institute,University of Miami Leonard M. Miller School of Medicine, 8Peggy and Harold Katz Family Drug Discovery Center,University of Miami Leonard M. Miller School of Medicine
Chapters
- 00:04Title
- 00:36Cell Seeding in 24-well Plate
- 02:54Live Cell Analysis
- 03:51Analysis Parameter Setup
- 05:41Analysis and Data Processing
- 07:06Results: Measuring LDL Cholesterol Uptake in Various Cells Using Live Cell Imaging
- 08:05Conclusion
Questo protocollo fornisce un approccio efficiente per misurare l'assorbimento del colesterolo di LDL con tariffe afflusso in tempo reale utilizzando una cellula viva imaging system in vari tipi cellulari. Questa tecnica fornisce una piattaforma per schermare l'attività farmacologica dei composti che interessano l'afflusso di LDL durante il monitoraggio per la morfologia delle cellule e quindi potenziale citotossicità.
