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LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring
 

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

Article DOI: 10.3791/58564-v • 08:45 min • November 17th, 2018
November 17th, 2018 •
*1,2, *1,3, 4, 4,5, 6, 1,2,7,8
1Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, 2Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, 3Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, 4Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami Leonard M. Miller School of Medicine, 5John P. Hussman Institute for Human Genomics, University of Miami Leonard M. Miller School of Medicine, 6Department of Medicine, Division of Endocrinology, Metabolism and Endocrinology and the Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, 7Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, 8Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine
* These authors contributed equally

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résumé
Automatic Translation

This protocol provides an efficient approach to measuring LDL cholesterol uptake with real time influx rates using a live cell imaging system in various cell types. This technique provides a platform to screen the pharmacological activity of compounds affecting LDL influx while monitoring for cell morphology and hence potential cytotoxicity.

Tags

LDL Cholesterol Uptake Assay Live Cell Imaging Analysis Cell Health Monitoring LDL Cholesterol Transport Toxic Compounds Detection Cell Morphology Monitoring Media Aspiration Cell Washing Trypsin-EDTA Solution Incubation At 37 Degrees Celsius Trypsin Neutralization Fetal Bovine Serum (FBS) Centrifugation Cell Resuspension Cell Counting Optimized Cell Density 24-well Plate Incubation
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