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An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis

An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis

DOI:
10.3791/58489-v

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07:26 min

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October 04, 2018

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, , , ,
1The Pirbright Institute

Chapters

  • 00:04Title
  • 00:45Isolation of Chicken Primary Bursal Cells
  • 02:59Culture of Chicken Primary Bursal Cells
  • 03:51Infection of Chicken Primary Bursal Cells with IBDV and Quantification of IBDV Replication
  • 05:02Results: Analysis of Chicken Primary Bursal Cell Culture and Infection with Infectious Bursal Disease Virus
  • 06:41Conclusion

Summary

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Here we describe the isolation of chicken primary bursal cells from the bursa of Fabricius, the culture and infection of the cells with infectious bursal disease virus, and the quantification of viral replication.

Tags

Ex VivoChickenPrimary Bursal-cell CultureInfectious Bursal Disease VirusPathogenesisAvian VirologyB CellsImmortalized Cell LinesThree RsPBSCollagenase DHBBSRPMIFBSDensity Gradient MediaPolysucroseSodium Diatrizoate

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