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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

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生物化学

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JoVE 杂志生物化学

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

6,965 Views

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06:01 min

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January 07, 2019

DOI:

10.3791/58451-v

DOI:

10.3791/58451-v

•

06:01 min

January 07, 2019

•

3 Views

Bushra Husain, Sairupa Paduchuri, Sree R. Ramani, Nadia Martinez-Martin

¹Receptor Discovery group, Microchemistry, Proteomics and Lipidomics Department,Genentech, ²Portfolio Management and Operations,Genentech

Tags

Extracellular Protein Microarray High Throughput Detection Low Affinity Receptor-ligand Interactions Protein-protein Interactions Micro Arrayer Epoxy Silane Slides Cy3 Labeled BSA Blocking Solution Multivalent Approach Fc-tagged Extracellular Domains Protein A Coated Microbeads Cy5 IgG Labeling

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