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Induksjon av Endothelial differensiering i Cardiac Progenitor celler Under lav Serum forhold
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions
DOI:

12:48 min

January 07, 2019

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Chapters

  • 00:04Title
  • 01:01Tissue Preparation and Digestion
  • 02:48Filtration
  • 04:43Primary Culture of Isolated SP-CPCs
  • 05:28SP-CPC Growth and Viability Under Different Serum Concentrations
  • 07:28Induction of Endothelial Differentiation
  • 08:59Tube Formation Assay
  • 10:15Results
  • 11:58Conclusion

Summary

Automatic Translation

Denne protokollen beskriver en endothelial differensiering teknikk for cardiac progenitor celler. Det fokuserer spesielt på hvordan serum konsentrasjonen og celle-såing tetthet påvirker potensial endothelial differensiering.

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