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Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions
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Biologie du développement
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Journal JoVE Biologie du développement
Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions

Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions

6,316 Views

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12:48 min

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January 07, 2019

DOI:

10.3791/58370-v

DOI:
10.3791/58370-v

•

12:48 min
January 07, 2019

•

2 Views
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1Department of Biomedicine,University Hospital and University of Basel, 2Division of Cardiology,University Hospital Basel

Summary

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This protocol describes an endothelial differentiation technique for cardiac progenitor cells. It particularly focuses on how serum concentration and cell-seeding density affect the endothelial differentiation potential.

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Cardiac Progenitor CellsEndothelial DifferentiationLow Serum ConditionsCell TherapyCardiac DiseaseAnesthetizeCollagenase BHank’s Balanced Salt SolutionRed Blood Cell Lysis Buffer

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Cite this Article

Mochizuki, M., Della Verde, G., Soliman, H., Pfister, O., Kuster, G. M. Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions. J. Vis. Exp. (143), e58370, doi:10.3791/58370 (2019).

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