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Hochdurchsatz-Messung der Plasmamembran Wiederverschließen Effizienz in Säugerzellen
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Immunology and Infection
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JoVE Journal Immunology and Infection
High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells
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Hochdurchsatz-Messung der Plasmamembran Wiederverschließen Effizienz in Säugerzellen

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7,597 Views

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10:07 min

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January 07, 2019

DOI:

10.3791/58351-v

DOI:
10.3791/58351-v

•

10:07 min
January 07, 2019

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1 Views
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1Department of Microbial Infection and Immunity,The Ohio State University, 2Department of Microbiology,The Ohio State University, 3Infectious Diseases Institute,The Ohio State University, 4Division of Biostatistics, College of Public Health,The Ohio State University

Summary

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Hier beschreiben wir eine Hochdurchsatz-Fluoreszenz basierende Assays, die die Plasmamembran Wiederverschließen Effizienz durch fluorometrisch und bildgebende Untersuchungen in lebenden Zellen misst. Dieser Test kann verwendet werden, für das screening von Drogen oder Zielgene, die Plasmamembran Wiederverschließen in Säugetierzellen zu regulieren.

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Cite this Article

Lam, J. G., Song, C., Seveau, S. High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells. J. Vis. Exp. (143), e58351, doi:10.3791/58351 (2019).

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