Journal
/
Immunologie et infection
/
High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

7,597 Views

•

10:07 min

•

January 07, 2019

DOI:

10.3791/58351-v

DOI:
10.3791/58351-v

•

10:07 min
January 07, 2019

•

1 Views
, ,
1Department of Microbial Infection and Immunity,The Ohio State University, 2Department of Microbiology,The Ohio State University, 3Infectious Diseases Institute,The Ohio State University, 4Division of Biostatistics, College of Public Health,The Ohio State University

Summary

Automatically generated

Here we describe a high-throughput fluorescence-based assay that measures the plasma membrane resealing efficiency through fluorometric and imaging analyses in living cells. This assay can be used for screening drugs or target genes that regulate plasma membrane resealing in mammalian cells.

Explore More Videos

Plasma Membrane ResealingHigh-throughputHeLa Cells96-well PlateFluorescencePropidium IodideKinetic AssayPlate ReaderOptical ConfigurationRead ModeRead Type

Read Article

Cite this Article

Lam, J. G., Song, C., Seveau, S. High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells. J. Vis. Exp. (143), e58351, doi:10.3791/58351 (2019).

Download .ris file

Copy

Share Video

.

Copy