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Fabbricazione di anisotropo polimerici artificiali Antigen Presenting Cells per l'attivazione delle cellule T CD8 +
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October 12, 2018
DOI:
1Biomedical Engineering, Translational Tissue Engineering Center, Institute for Nanobiotechnology,Johns Hopkins University School of Medicine, 2Biomedical Engineering, Translational Tissue Engineering Center, Institute for Nanobiotechnology, Ophthalmology, Oncology, Neurosurgery, Materials Science and Engineering, Chemical and Biomolecular Engineering, and the Bloomberg-Kimmel Institute for Cancer Immunotherapy,Johns Hopkins University School of Medicine
Summary
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Qui, presentiamo un protocollo per in modo rapido e riproducibile generare articifical biologicamente ispirato, biodegradabile antigene che presenta le cellule (aAPC) con sintonizzabile dimensione, forma e presentazione di proteina di superficie per cellula T espansione ex vivo o in vivo .
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Ben-Akiva, E., Rhodes, K. R., Meyer, R. A., Green, J. J. Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation. J. Vis. Exp. (140), e58332, doi:10.3791/58332 (2018).
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