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Un Cell-based Assay pour étudier la myosine Non musculaire II contractilité via la gastrulation plié voie de signalisation de cellules S2R + Drosophila
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells
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Un Cell-based Assay pour étudier la myosine Non musculaire II contractilité via la gastrulation plié voie de signalisation de cellules S2R + Drosophila

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7,227 Views

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07:15 min

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August 19, 2018

DOI:

10.3791/58325-v

DOI:
10.3791/58325-v

•

07:15 min
August 19, 2018

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1 Views
, , , , , , ,
1Department of Biology,The University of North Carolina at Chapel Hill, 2Department of Biology,Reed College, 3Carolina Center for Genome Sciences,The University of North Carolina at Chapel Hill, 4Lineberger Comprehensive Cancer Center,The University of North Carolina at Chapel Hill

Summary

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Nous décrivons ici un essai de contractilité S2R + cellules de drosophile . L’application d’un ligand exogène, gastrulation plié (Fog), conduit à l’activation du brouillard contractilité cellulaire et de la voie de signalisation. Ce test peut être utilisé pour étudier la régulation des protéines cellulaires contractilité dans le brouillard, voie de signalisation.

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Cite this Article

Peters, K. A., Detmar, E., Sepulveda, L., Del Valle, C., Valsquier, R., Ritz, A., Rogers, S. L., Applewhite, D. A. A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells. J. Vis. Exp. (138), e58325, doi:10.3791/58325 (2018).

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