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A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells

A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells

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07:15 min

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August 19, 2018

DOI:

10.3791/58325-v

DOI:
10.3791/58325-v

•

07:15 min
August 19, 2018

•

1 Views
, , , , , , ,
1生物学科,The University of North Carolina at Chapel Hill, 2生物学科,Reed College, 3Carolina Center for Genome Sciences,The University of North Carolina at Chapel Hill, 4Lineberger Comprehensive Cancer Center,The University of North Carolina at Chapel Hill

概要

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Here we describe a contractility assay using Drosophila S2R+ cells. The application of an exogenous ligand, folded gastrulation (Fog), leads to the activation of the Fog signaling pathway and cellular contractility. This assay can be used to investigate the regulation of cellular contractility proteins in the Fog signaling pathway.

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Cite this Article

Peters, K. A., Detmar, E., Sepulveda, L., Del Valle, C., Valsquier, R., Ritz, A., Rogers, S. L., Applewhite, D. A. A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells. J. Vis. Exp. (138), e58325, doi:10.3791/58325 (2018).

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