Journal
/
Immunology and Infection
/
利用超滤离心技术分离小鼠支气管肺泡灌洗液中的细胞外囊
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique
Please note that all translations are automatically generated. Click here for the English version.

利用超滤离心技术分离小鼠支气管肺泡灌洗液中的细胞外囊

DOI:
10.3791/58310-v

•

09:18 min

•

November 09, 2018

•

, , ,
1Department of Medicine, Division of Pulmonary and Critical Care, Women’s Guild Lung Institute,Cedars-Sinai Medical Center, 2Department of Biomedical Sciences,Cedars-Sinai Medical Center, 3Department of Medicine, Smidt Heart Institute,Cedars-Sinai Medical Center

Chapters

  • 00:04Title
  • 01:15Murine Bronchoalveolar Lavage Fluid (BALF) Collection and Preparation
  • 02:21Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid
  • 04:56Extracellular Vesicle Quantification
  • 05:35Detection of BALF-derived Extracellular Vesicles
  • 07:16Results: UFC Method Demonstrates Higher Total Particle Counts and Protein Recover
  • 08:25Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们描述了两个细胞外囊泡分离方案, 超滤离心和超离心与密度梯度离心, 从小鼠支气管肺泡灌洗液样品中分离细胞外囊泡。用两种方法对小鼠支气管肺泡灌洗液中的细胞外囊泡进行了定量和表征。

Tags

Extracellular VesiclesBronchoalveolar Lavage FluidUltrafiltration CentrifugationUltracentrifugationDensity Gradient PurificationMurineTracheaDPBSSupernatantSyringe FilterCentrifugal Filter UnitRetentateAliquot

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

皮层小胶质细胞的分离与保存免疫表型和功能从新生儿鼠

口咽部气管内PAMP管理和支气管肺泡灌洗的利用率来评估小鼠宿主的免疫反应

隔离和小鼠骨髓的静脉注射单核细胞衍生

从小鼠组织在母胎界面分离白细胞

分离及小鼠淋巴细胞的激活

流量Virometry来分析病毒颗粒的抗原谱和外囊泡

支气管肺泡灌洗小鼠肺分析炎症细胞浸润

成年日本血吸虫细胞外囊泡的分离与鉴定

用于阿尔韦拉尔巨噬细胞和CD4和T细胞免疫造血和HIV储液评估的支气管性洗浴液和匹配血液的处理

使用胶原酶E从毛里结肠分离拉米纳普里亚单核细胞

Read Article