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In Vitro Ricostituzione di Self-Organizing pattern proteico su lipidici supportati
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
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In Vitro Ricostituzione di Self-Organizing pattern proteico su lipidici supportati

italiano

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11,754 Views

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08:10 min

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July 28, 2018

DOI:

10.3791/58139-v

DOI:
10.3791/58139-v

•

08:10 min
July 28, 2018

•

4 Views
, ,
1Department of Cellular and Molecular Biophysics,Max Planck Institute of Biochemistry

Summary

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Forniamo un protocollo per in vitro test di auto-organizzazione della mente e la mia su un doppio strato lipidico supportati in una camera aperta. Inoltre, descriviamo come racchiudere il dosaggio del lipido-clad PDMS microcompartments per simulare condizioni in vivo di confinamento di reazione.

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Cite this Article

Ramm, B., Glock, P., Schwille, P. In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers. J. Vis. Exp. (137), e58139, doi:10.3791/58139 (2018).

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