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In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers

In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers

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11,763 Views

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08:10 min

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July 28, 2018

DOI:

10.3791/58139-v

DOI:
10.3791/58139-v

•

08:10 min
July 28, 2018

•

4 Views
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1Department of Cellular and Molecular Biophysics,Max Planck Institute of Biochemistry

Summary

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We provide a protocol for in vitro self-organization assays of MinD and MinE on a supported lipid bilayer in an open chamber. Additionally, we describe how to enclose the assay in lipid-clad PDMS microcompartments to mimic in vivo conditions by reaction confinement.

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Cite this Article

Ramm, B., Glock, P., Schwille, P. In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers. J. Vis. Exp. (137), e58139, doi:10.3791/58139 (2018).

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