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Genetics
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分離の特性と人間の歯小嚢細胞のマイクロ Rna を用いた遺伝子組み換え
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Genetics
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JoVE Journal
Genetics
Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
分離の特性と人間の歯小嚢細胞のマイクロ Rna を用いた遺伝子組み換え
DOI:
10.3791/58089-v
•
07:56 min
•
November 16, 2018
•
Paula Müller*
1,2
,
Katharina Ekat*
3
,
Anne Brosemann
,
Anne Köntges
,
Robert David
2
,
Hermann Lang
1
Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery
,
Rostock University Medical Center
,
2
Department Life
,
Light and Matter of the Interdisciplinary Faculty at Rostock University
,
3
Department of Operative Dentistry and Periodontology
,
Rostock University Medical Center
Chapters
00:04
Title
00:33
Isolation of hDFSCs
02:50
Transfection of hDFSCs
04:07
Analysis of Transfection
06:02
Results: Successful Isolation and Genetic Modification of hDFSC
07:37
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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italiano (Italian)
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Automatic Translation
このプロトコルでは、人間の歯小嚢から抽出された歯の幹細胞の一時的な遺伝子工学について説明します。適用の非ウイルス性変更戦略は治療幹細胞製品の改善のための基礎になることがあります。
Tags
Dental Follicle Stem Cells
HDFSCs
Enzymatic Digestion
Collagenase
Dispase
MicroRNA
Genetic Modification
Regenerative Medicine
Cell Isolation
Cell Characterization
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