Journal
/
遗传学
/
Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
JoVE 杂志
遗传学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 遗传学
Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

DOI:
10.3791/58089-v

•

07:56 min

•

November 16, 2018

•

, , , , ,
1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery,Rostock University Medical Center, 2Department Life,Light and Matter of the Interdisciplinary Faculty at Rostock University, 3Department of Operative Dentistry and Periodontology,Rostock University Medical Center

Chapters

  • 00:04Title
  • 00:33Isolation of hDFSCs
  • 02:50Transfection of hDFSCs
  • 04:07Analysis of Transfection
  • 06:02Results: Successful Isolation and Genetic Modification of hDFSC
  • 07:37Conclusion

Summary

自动翻译

自动翻译

This protocol describes the transient genetic engineering of dental stem cells extracted from the human dental follicle. The applied non-viral modification strategy may become a basis for the improvement of therapeutic stem cell products.

Tags

Dental Follicle Stem CellsHDFSCsEnzymatic DigestionCollagenaseDispaseMicroRNAGenetic ModificationRegenerative MedicineCell IsolationCell Characterization

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

Read Article