Journal
/
Immunology and Infection
/
In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells

In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells

English

Automatically Generated

7,040 Views

•

10:27 min

•

September 10, 2018

DOI:

10.3791/58087-v

DOI:
10.3791/58087-v

•

10:27 min
September 10, 2018

•

1 Views
, ,
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 2Immunology Programme, Life Sciences Institute,National University of Singapore, 3Cancer Science Institute of Singapore, Yong Loo Lin School of Medicine,National University of Singapore

Summary

Automatically generated

Here, we present a protocol to differentiate murine granulocyte-macrophage-colony-stimulating-factor-producing T helper (THGM) cells from naive CD4+ T cells, including isolation of naive CD4+ T cells, differentiation of THGM, and analysis of differentiated THGM cells. This method can be applied to studies of the regulation and function of THGM cells.

Explore More Videos

CD4 T-cellsGM-CSFT Helper CellsTHGM CellsIn Vitro DifferentiationInflammatory DiseasesMultiple SclerosisCell IsolationCell CultureMagnetic Separation

Read Article

Cite this Article

Lu, Y., Fu, X., Zhang, Y. In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells. J. Vis. Exp. (139), e58087, doi:10.3791/58087 (2018).

Download .ris file

Copy

Share Video

.

Copy