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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells
 

In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells

Article DOI: 10.3791/58087-v • 10:28 min • September 10th, 2018
September 10th, 2018 •
1,2,3, 3, 1,2
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Immunology Programme, Life Sciences Institute, National University of Singapore, 3Cancer Science Institute of Singapore, Yong Loo Lin School of Medicine, National University of Singapore

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Here, we present a protocol to differentiate murine granulocyte-macrophage-colony-stimulating-factor-producing T helper (THGM) cells from naive CD4+ T cells, including isolation of naive CD4+ T cells, differentiation of THGM, and analysis of differentiated THGM cells. This method can be applied to studies of the regulation and function of THGM cells.

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In Vitro Differentiation Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF) T Helper (THGM) Cells CD4 T-cell Field Subset Of T-helper Cells Inflammatory Diseases Multiple Sclerosis Technique GM-CSF Expressing T-cells Naive CD4 T-cells Gamma OI17 Expression Cells Cellular Source Regression And Function Demonstration Yi Lu Graduate Student Forceps Abdomen Incision Peritoneal Sac Spleen Cell Isolation Buffer Syringe Maceration Cell Collection Centrifugation
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