JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Chemistry
/
用快速 Bioorthogonal 反应生成抗体-药物共轭物的非规范氨基酸的遗传编码
JoVE Journal
Chemistry
This content is Free Access.
JoVE Journal
Chemistry
Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction
Please note that all translations are automatically generated.
Click here for the English version.
用快速 Bioorthogonal 反应生成抗体-药物共轭物的非规范氨基酸的遗传编码
DOI:
10.3791/58066-v
•
11:02 min
•
September 14, 2018
•
Benjamí Oller-Salvia
1
Medical Research Council Laboratory of Molecular Biology
Chapters
00:04
Title
00:30
Produce and Characterize the Antibody
03:47
Conjugate the Antibody and Characterize the Antibody-Drug Conjugates (ADC)
06:38
Assay Stability of the Dihydropyridazine Linkage in Trastuzumab(TAMRA)
2
in Serum
07:46
Assess the Cytotoxicity of the ADC
09:11
Results: Antibody-Drug Conjugate is Stable, Potent, and Selective
10:31
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
将赖氨酸的 cyclopropene 衍生物加入抗体, 就能使四嗪类分子的特异性、快速和有效的联系产生抗体-药物共轭。
Tags
Genetic Encoding
Non-canonical Amino Acid
Antibody-drug Conjugates
Bioorthogonal Reaction
Protein Modification
Antibody Production
CpK
HEK Cells
Protein A Purification
FPLC
Hydrophobic Interaction Chromatography
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
二碘化钐的制备和使用(SMI<sub> 2</sub>)在有机合成中:HMPA和镍(II)盐的钐巴尔比耶反应的机理作用
Preparation of Silica Nanoparticles Through Microwave-assisted Acid-catalysis
表面修饰碳纳米管通过土柱运输
洞察氨基酸和多肽与无机材料的使用相互作用的单分子力谱
制备和评价<sup>99米</sup>锝标记的三齿螯合物预靶向使用生物正交化学
对碳化钼催化合成乙酸的程序升温脱氧
连续流动化学: Diphenyldiazomethane 与
p
硝基苯甲酸的反应
酶联反应合成 l-赖氨酸手性氨基醇的研究
生物哺乳动物细胞光交联图谱和 Bioorthogonal 化学中化学探针的遗传受体优化
第三代机械 Vaping 装置生成电子烟气溶胶: 毒理学研究的应用
Read Article