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Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction
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Chemistry
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JoVE Journal Chemistry
Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction

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7,472 Views

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11:02 min

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September 14, 2018

DOI:

10.3791/58066-v

DOI:
10.3791/58066-v

•

11:02 min
September 14, 2018

•

10 Views
1Medical Research Council Laboratory of Molecular Biology

Summary

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Incorporating a cyclopropene derivative of lysine into antibodies allows the site-specific, rapid and efficient linkage of tetrazine-bearing molecules to generate antibody-drug conjugates.

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Genetic EncodingNon-canonical Amino AcidAntibody-drug ConjugatesBioorthogonal ReactionProtein ModificationAntibody ProductionCpKHEK CellsProtein A PurificationFPLCHydrophobic Interaction Chromatography

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Oller-Salvia, B. Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction. J. Vis. Exp. (139), e58066, doi:10.3791/58066 (2018).

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