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バイオアッセイ誘導分別を使用して海ヤツメウナギ フェロモンの同定
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation
DOI:

09:35 min

July 17, 2018

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Chapters

  • 00:04Title
  • 00:27Collection and Extraction of Sea Lamprey Conditioned Water
  • 01:14Isolation of Fraction Pools with Chromatography
  • 02:51Electro-olfactogram Recordings to Identify Odorous Fractions/Compounds
  • 05:43Two-choice Maze Behavioral Bioassay to Identify Behaviorally Active Fractions/Compounds
  • 06:31Chromatographic Isolation of Pure Compounds from Active Fractions
  • 06:58Structure Elucidation of a Pure Compound with Mass Spectrometry and Nuclear Magnetic Resonance
  • 07:58Results: Behavioral Responses of Ovulated Female Sea Lampreys to Odorants
  • 08:36Conclusion

Summary

Automatic Translation

ここでは、分離し、構造、嗅力海ヤツメウナギの推定フェロモン化合物の行動応答を評価するためのプロトコルを提案する.

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