Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions
7,474 Views
•
07:20 min
•
August 31, 2018
DOI:
Summary
Automatically generated
The aphid Aphis nerii colonizes on highly-defended plants in the dogbane family (Apocyanaceae) and provides numerous opportunities to study plant-insect interactions. Here, we present a series of protocols for the maintenance of plant and aphid cultures, and the generation and analysis of molecular and -omic data for A. nerii.
Read Article
Cite this Article
Birnbaum, S. S., Rinker, D. C., Abbot, P. Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions. J. Vis. Exp. (138), e58044, doi:10.3791/58044 (2018).
Copy