Journal
/
Environment
/
Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
JoVE Journal
Environment
This content is Free Access.
JoVE Journal Environment
Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

English

Automatically Generated

11,520 Views

•

09:57 min

•

July 12, 2018

DOI:

10.3791/58033-v

DOI:
10.3791/58033-v

•

09:57 min
July 12, 2018

•

9 Views
, , ,
1Department of Environmental Microbiology,Helmholtz Centre for Environmental Research – UFZ

Summary

Automatically generated

Flow cytometric analysis has proven valuable for investigating pure cultures and monitoring microbial community dynamics. We present three comprehensive workflows, from sampling to data analysis, for pure cultures and complex communities in clear medium as well as in challenging matrices, respectively.

Explore More Videos

Microbiome DynamicsFlow CytometryMicrobial EcologyBiotechnologyNatural CommunitiesBiogas CommunityActivated SludgeCell FixationCell StabilizationPBSFormaldehydeEthanolSonicationOD 700Permeabilization Buffer

Read Article

Cite this Article

Lambrecht, J., Schattenberg, F., Harms, H., Mueller, S. Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities. J. Vis. Exp. (137), e58033, doi:10.3791/58033 (2018).

Download .ris file

Copy

Share Video

.

Copy