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Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
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Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

11,520 Views

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09:57 min

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July 12, 2018

DOI:

10.3791/58033-v

DOI:
10.3791/58033-v

•

09:57 min
July 12, 2018

•

9 Views
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1Department of Environmental Microbiology,Helmholtz Centre for Environmental Research – UFZ

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Flow cytometric analysis has proven valuable for investigating pure cultures and monitoring microbial community dynamics. We present three comprehensive workflows, from sampling to data analysis, for pure cultures and complex communities in clear medium as well as in challenging matrices, respectively.

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Lambrecht, J., Schattenberg, F., Harms, H., Mueller, S. Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities. J. Vis. Exp. (137), e58033, doi:10.3791/58033 (2018).

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