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Phosphopeptide Bereicherung gepaart mit quantitativen markierungsfreie Massenspektrometrie zu untersuchen, die Phosphoproteome bei Prostatakrebs
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August 02, 2018
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1Graduate Program in Cellular and Molecular Pharmacology, School of Graduate Studies,Rutgers University, The State University of New Jersey, 2Graduate Program in Quantitative Biomedicine, School of Graduate Studies,Rutgers University, The State University of New Jersey, 3Department of Medicine, Division of Medical Oncology,Rutgers Robert Wood Johnson Medical School, 4Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, Jonsson Comprehensive Cancer Center, UCLA Metabolomics Center, and California NanoSystems Institute,David Geffen School of Medicine, University of California, Los Angeles, 5Mork Family Department of Chemical Engineering and Materials Science,University of Southern California, 6Pharmacology,Rutgers Robert Wood Johnson Medical School, 7Cancer Metabolism and Growth Program,Rutgers Cancer Institute of New Jersey
Summary
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Dieses Protokoll beschreibt ein Verfahren zum Extrahieren und Phosphopeptides von Prostatakrebs-Zelllinien oder Gewebe für eine Analyse der Phosphoproteome per Massenspektrometrie-basierte Proteomics zu bereichern.
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Cheng, L. C., Li, Z., Graeber, T. G., Graham, N. A., Drake, J. M. Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer. J. Vis. Exp. (138), e57996, doi:10.3791/57996 (2018).
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