This protocol describes a procedure to extract and enrich phosphopeptides from prostate cancer cell lines or tissues for an analysis of the phosphoproteome via mass spectrometry-based proteomics.
Cheng, L. C., Li, Z., Graeber, T. G., Graham, N. A., Drake, J. M. Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer. J. Vis. Exp. (138), e57996, doi:10.3791/57996 (2018).