Journal
/
Neuroscience
/
小鼠原代多元体的体外模拟？
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons
Please note that all translations are automatically generated. Click here for the English version.

小鼠原代多元体的体外模拟？

English

Automatically Generated

6,833 Views

•

07:43 min

•

January 07, 2019

DOI:

10.3791/57988-v

DOI:
10.3791/57988-v

•

07:43 min
January 07, 2019

•

1 Views
, ,
1Institut NeuroMyoGène, CNRS UMR5310, INSERM U1217,Université Lyon, 2Unité Fonctionnelle de Neurogénétique Moléculaire,Hospices Civils de Lyon, 3Centre de Biotechnologie Cellulaire,Hospices Civils de Lyon

Summary

Automatically generated

这项技术的目的是准备一种高度丰富的来自小鼠脊髓的原发运动神经元 (mns) 培养。为了评估引起 mn 疾病的突变的后果, 我们在这里描述了这些孤立的 m2 的隔离和它们通过磁光的转染。

Explore More Videos

Charcot-Marie-Tooth DiseaseIn Vitro ModelingPrimary Motor NeuronsTransfectionNeurobiologyMotor Neuron PolarityAxon GuidanceAxon TraffickingNeurodegenerative MechanismsEnriched Motor Neuron CultureKnockdown ProteinSpinal Cord DissectionEnzymatic DigestionCell SuspensionDensity Gradient CentrifugationMotoneuron Enrichment

Read Article

Cite this Article

Jacquier, A., Risson, V., Schaeffer, L. Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons. J. Vis. Exp. (143), e57988, doi:10.3791/57988 (2019).

Download .ris file

Copy

Share Video

.

Copy