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Saccharomyces cerevisiae Metabolik 4-thiouracil ve bir miktar ile etiketleme yeni mRNA RNA polimeraz II etkinlik için bir Proxy olarak sentezlenmiş
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Genetics
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JoVE Journal Genetics
Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
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Saccharomyces cerevisiae Metabolik 4-thiouracil ve bir miktar ile etiketleme yeni mRNA RNA polimeraz II etkinlik için bir Proxy olarak sentezlenmiş

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8,872 Views

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09:21 min

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October 22, 2018

DOI:

10.3791/57982-v

DOI:
10.3791/57982-v

•

09:21 min
October 22, 2018

•

9 Views
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1Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France, 2Centre National de la Recherche Scientifique, Illkirch, France, 3Institut National de la Santé et de la Recherche Médicale, Illkirch, France, 4Université de Strasbourg

Summary

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Burada açıklanan protokolü yeni sentezlenmiş mRNA 4-thiouracil ile etiketli Maya hücrelerinden saf genom çapında miktar temel alır. Bu yöntem mRNA decay edilişi mRNA sentezi ölçmek için sağlar ve böylece, RNA polimeraz II transkripsiyon doğru bir ölçüm sağlar.

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Baptista, T., Devys, D. Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity. J. Vis. Exp. (140), e57982, doi:10.3791/57982 (2018).

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