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Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine
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Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine

Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine

English

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10,829 Views

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08:33 min

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July 24, 2018

DOI:

10.3791/57938-v

DOI:
10.3791/57938-v

•

08:33 min
July 24, 2018

•

15 Views
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1Department of Environmental Science, Center for Reservoir and Aquatic Systems Research,Baylor University, 2Institute of Biomedical Studies,Baylor University

Summary

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Here, we present a protocol to examine larval zebrafish and fathead minnow locomotor activities and photomotor responses (PMR) using an automated tracking software. When incorporated in common toxicology bioassays, analyses of these behaviors provide a diagnostic tool to examine chemical bioactivity. This protocol is described using caffeine, a model neurostimulant.

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Fish ModelsBehavioral ResponseLarval FishCaffeineNeuro-stimulantEnvironmental Biomedical SciencesLocomotor ActivityPhotomotor ResponseZebrafishFathead MinnowExposure ChambersVideo Tracking

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Steele, W. B., Mole, R. A., Brooks, B. W. Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine. J. Vis. Exp. (137), e57938, doi:10.3791/57938 (2018).

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