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Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish
 

Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine

Article DOI: 10.3791/57938-v • 08:33 min • July 24th, 2018
July 24th, 2018 •
1,2, 1, 1,2
1Department of Environmental Science, Center for Reservoir and Aquatic Systems Research, Baylor University, 2Institute of Biomedical Studies, Baylor University

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Here, we present a protocol to examine larval zebrafish and fathead minnow locomotor activities and photomotor responses (PMR) using an automated tracking software. When incorporated in common toxicology bioassays, analyses of these behaviors provide a diagnostic tool to examine chemical bioactivity. This protocol is described using caffeine, a model neurostimulant.

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Experimental Protocol Behavioral Response Profiles Larval Fish Neuro-stimulant Caffeine Fish Models Biomedical Sciences Drug Discovery Toxicology Studies Environmental Research Aquatic Toxicology Behavioral Ecology Chemicals Bioactivities Hazards Sensitive Approach Rapid Approach Environmental And Biomedical Sciences Commercial Bioactivities Behavioral Effects Pharmaceuticals Pesticides Comparative Environmental Pharmacology Toxicology Data Industrial Compounds Larval Locomotor Activity Light-dark Photo Periods Larval Photomotor Responses
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