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线虫线虫中乳酸和丙酮酸细胞的小尺度比色测定
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Biochemistry
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JoVE Journal Biochemistry
Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans
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线虫线虫中乳酸和丙酮酸细胞的小尺度比色测定

English

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6,781 Views

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09:35 min

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October 15, 2018

DOI:

10.3791/57807-v

DOI:
10.3791/57807-v

•

09:35 min
October 15, 2018

•

16 Views
, ,
1Department of Health Science, School of Sports & Health Science,Daito Bunka University, 2Department of Molecular Life Science, School of Medicine,Tokai University, 3Department of Health Management, School of Health Study,Tokai University

Summary

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本文介绍了一种改良的小尺度萃取和比色法测定乳酸和丙酮酸在线虫中的含量.使用商用试剂盒时, 其灵敏度和准确度的技术发展非常重要。在萃取中蛋白质沉淀是定量测定细胞内代谢产物的关键步骤。

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Yanase, S., Yasuda, K., Ishii, N. Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans. J. Vis. Exp. (140), e57807, doi:10.3791/57807 (2018).

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