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Efficiënte reiniging en LC-MS/MS-based Assay ontwikkeling voor tien-elf translocatie-2 5-Methylcytosine Dioxygenase
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Biochemistry
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JoVE Journal Biochemistry
Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
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Efficiënte reiniging en LC-MS/MS-based Assay ontwikkeling voor tien-elf translocatie-2 5-Methylcytosine Dioxygenase

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8,067 Views

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10:33 min

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October 15, 2018

DOI:

10.3791/57798-v

DOI:
10.3791/57798-v

•

10:33 min
October 15, 2018

•

1 Views
, , , ,
1Division of Pharmaceutical Sciences, School of Pharmacy,University of Missouri-Kansas City

Summary

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Hier presenteren we een protocol voor een efficiënte Macrostap zuivering van de actieve niet-gelabelde mens tien-elf translocatie-2 (TET2) 5-methylcytosine-dioxygenase met behulp van ionenwisselingschromatografie- en haar kwantitatieve analyse met behulp van een vloeibare chromatografie-tandem-massa spectrometrie (LC-MS/MS)-gebaseerde aanpak.

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Cite this Article

Bhattacharya, C., Dey, A. S., Ayon, N. J., Gutheil, W. G., Mukherji, M. Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase. J. Vis. Exp. (140), e57798, doi:10.3791/57798 (2018).

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