Journal
/
Biochemistry
/
Etkili arınma ve on-Eleven Translocation-2 5-metilsitozin Dioxygenase için tahlil LC-MS/MS tabanlı geliştirme
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
Please note that all translations are automatically generated. Click here for the English version.

Etkili arınma ve on-Eleven Translocation-2 5-metilsitozin Dioxygenase için tahlil LC-MS/MS tabanlı geliştirme

Türkçe

Automatically Generated

8,067 Views

•

10:33 min

•

October 15, 2018

DOI:

10.3791/57798-v

DOI:
10.3791/57798-v

•

10:33 min
October 15, 2018

•

1 Views
, , , ,
1Division of Pharmaceutical Sciences, School of Pharmacy,University of Missouri-Kansas City

Summary

Automatically generated

Burada, on-on translocation-2 (TET2) iletişim kuralı etkin etiketsiz insan bir verimli tek adım arıtma için mevcut iyon değiştirme Kromatografi ve sıvı kromatografi-tandem mass kullanarak onun tahlil kullanarak 5-metilsitozin dioxygenase spektrometresi (LC-MS/MS)-yaklaşım alarak.

Explore More Videos

TET25-Methylcytosine DioxygenaseProtein PurificationLC-MS/MSBacterial TransformationProtein ExpressionIPTG InductionE. Coli BL21 DE3EpigeneticsDemethylation

Read Article

Cite this Article

Bhattacharya, C., Dey, A. S., Ayon, N. J., Gutheil, W. G., Mukherji, M. Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase. J. Vis. Exp. (140), e57798, doi:10.3791/57798 (2018).

Download .ris file

Copy

Share Video

.

Copy