Journal
/
Biochemistry
/
Toewijzing metabolisme: Bewakingsactiviteit lactaat Dehydrogenase rechtstreeks in weefsel
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
Please note that all translations are automatically generated. Click here for the English version.

Toewijzing metabolisme: Bewakingsactiviteit lactaat Dehydrogenase rechtstreeks in weefsel

Nederlands

Automatically Generated

11,102 Views

•

06:18 min

•

June 21, 2018

DOI:

10.3791/57760-v

DOI:
10.3791/57760-v

•

06:18 min
June 21, 2018

•

10 Views
, , , , , , , ,
1Department of Molecular, Cell and Developmental Biology,UCLA, 2Department of Biological Chemistry,David Geffen School of Medicine, 3Molecular Biology Institute Interdepartmental Program,UCLA

Summary

Automatically generated

We beschrijven een protocol voor het toewijzen van de ruimtelijke spreiding van enzymatische activiteit voor enzymen die nicotinatmide adenine dinucleotide-fosfaat (NAD(P)H) + H + rechtstreeks in weefselmonsters genereren.

Explore More Videos

Lactate DehydrogenaseEnzyme ActivityCryomoldCryosectioningHistochemical StainingNADPMSNitrotetrazolium Blue ChlorideLactateEnzyme Localization

Read Article

Cite this Article

Jelinek, D., Flores, A., Uebelhoer, M., Pasque, V., Plath, K., Iruela-Arispe, M. L., Christofk, H. R., Lowry, W. E., Coller, H. A. Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue. J. Vis. Exp. (136), e57760, doi:10.3791/57760 (2018).

Download .ris file

Copy

Share Video

.

Copy