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Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
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生物化学
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Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue

11,098 Views

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06:18 min

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June 21, 2018

DOI:

10.3791/57760-v

DOI:
10.3791/57760-v

•

06:18 min
June 21, 2018

•

10 Views
, , , , , , , ,
1Department of Molecular, Cell and Developmental Biology,UCLA, 2Department of Biological Chemistry,David Geffen School of Medicine, 3Molecular Biology Institute Interdepartmental Program,UCLA

Summary

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We describe a protocol for mapping the spatial distribution of enzymatic activity for enzymes that generate nicotinatmide adenine dinucleotide phosphate (NAD(P)H) + H+ directly in tissue samples.

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Jelinek, D., Flores, A., Uebelhoer, M., Pasque, V., Plath, K., Iruela-Arispe, M. L., Christofk, H. R., Lowry, W. E., Coller, H. A. Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue. J. Vis. Exp. (136), e57760, doi:10.3791/57760 (2018).

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