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Electroquímico detección de deuterio cinético isótopo efecto en extracelular transporte de electrones en la Shewanella oneidensis MR-1
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Biochemistry
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JoVE Journal Biochemistry
Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
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Electroquímico detección de deuterio cinético isótopo efecto en extracelular transporte de electrones en la Shewanella oneidensis MR-1

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9,784 Views

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09:00 min

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April 16, 2018

DOI:

10.3791/57584-v

DOI:
10.3791/57584-v

•

09:00 min
April 16, 2018

•

4 Views
, ,
1Department of Applied Chemistry,University of Tokyo, 2Global Research Center for Environment and Energy based on Nanomaterials Science,National Institute for Materials Science

Summary

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Aquí presentamos un protocolo de los experimentos electroquímicos celulares para estudiar la contribución del transporte de protones a la velocidad de transporte de electrones extracelular a través de los citocromos de la membrana externa de Shewanella oneidensis MR-1.

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Tokunou, Y., Hashimoto, K., Okamoto, A. Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1. J. Vis. Exp. (134), e57584, doi:10.3791/57584 (2018).

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