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Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
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Biochimie
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Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

9,784 Views

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09:00 min

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April 16, 2018

DOI:

10.3791/57584-v

DOI:
10.3791/57584-v

•

09:00 min
April 16, 2018

•

4 Views
, ,
1Department of Applied Chemistry,University of Tokyo, 2Global Research Center for Environment and Energy based on Nanomaterials Science,National Institute for Materials Science

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Here we present a protocol of whole-cell electrochemical experiments to study the contribution of proton transport to the rate of extracellular electron transport via the outer-membrane cytochromes complex in Shewanella oneidensis MR-1.

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Deuterium Kinetic Isotope EffectExtracellular Electron TransportShewanella Oneidensis MR-1Electrochemical DetectionProton TransportC-type CytochromesThree-electrode Electrochemical ReactorITO SubstrateAnaerobic ConditionsLactateYeast Extract

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Tokunou, Y., Hashimoto, K., Okamoto, A. Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1. J. Vis. Exp. (134), e57584, doi:10.3791/57584 (2018).

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