Journal
/
Inmunología y contagio
/
Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages
JoVE Revista
Inmunología y contagio
This content is Free Access.
JoVE Revista Inmunología y contagio
Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

DOI:
10.3791/57566-v

•

07:24 min

•

October 19, 2018

•

, , ,
1Institut für Molekulare Zellbiologie

Capítulos

  • 00:04Título
  • 01:00Resident Mouse Peritoneal Macrophage and Human Red Blood Cell Isolation
  • 04:00Macrophage Plasma Membrane Labeling and Human Red Blood Cell-mouse Immunoglobulin (IgG) Opsonization and Imaging
  • 06:32Results: Representative Time-lapse, 3D-imaging of Phagocytosis
  • 07:02Conclusion

Summary

Traducción Automática

Traducción Automática

Here we describe methods using spinning disk confocal microscopy to image single phagocytic events by mouse resident peritoneal macrophages. The protocols can be extended to other phagocytic cells.

Tags

PhagocytosisMacrophagesTime-lapse3D ImagingCell IsolationPeritoneal LavageRPMI MediumHEPESFibronectin-coated SlidesAir Bubble RemovalCell IncubationMicroscopy

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

Proteomic Profiling of Macrophages by 2D Electrophoresis

Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device

Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

Read Article