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Biochemistry
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C2C12 Myotubes 测量反式等离子体膜电子传输
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes
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C2C12 Myotubes 测量反式等离子体膜电子传输
DOI:
10.3791/57565-v
•
10:27 min
•
May 04, 2018
•
Shannon C. Kelly*
1
,
Amanda M. Eccardt*
1
,
Jonathan S. Fisher
1
Department of Biology
,
Saint Louis University
Chapters
00:04
Title
00:39
WST-1 Reduction Assay
04:23
DPIP Reduction Assay
06:17
Determination of Whether Reduced Electron Acceptors are Substrates for AO or SOD
07:59
Results: Measuring Trans-Plasma Membrane Electron Transport
09:34
Conclusion
Summary
Automatic Translation
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Automatic Translation
本协议的目标是通过细胞外电子受体 spectrophotometrically 监测反式等离子体膜电子传输, 并分析这些细胞外电子受体可能发生的酶相互作用。
Tags
C2C12 Myotubes
Trans-plasma Membrane Electron Transport
Spectrophotometric Assay
WST-1
PMS
Glucose
PBS
Real-time Monitoring
Redox Biology
Cell Culture
Differentiation
Absorbance Measurement
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