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Yerli, etiketsiz Huntingtin Exon1 Monomer ve SUMO Fusion strateji kullanarak liflerinde nesil
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy
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Yerli, etiketsiz Huntingtin Exon1 Monomer ve SUMO Fusion strateji kullanarak liflerinde nesil

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7,655 Views

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11:22 min

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June 27, 2018

DOI:

10.3791/57506-v

DOI:
10.3791/57506-v

•

11:22 min
June 27, 2018

•

3 Views
, , ,
1Laboratory of Molecular and Chemical Biology of Neurodegeneration,Ecole Polytechnique Fédérale de Lausanne (EPFL)

Summary

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Burada, yerli, etiket içermeyen monomerleri miktarlarda miligram ve liflerinde küçük Ubikuitin ilgili değiştirici (SUMO) geçici fusion tabanlı exon1 Huntingtin protein (Httex1) üretimi için sağlam ve en iyi duruma getirilmiş iletişim kuralı mevcut.

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Cite this Article

Reif, A., Chiki, A., Ricci, J., Lashuel, H. A. Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy. J. Vis. Exp. (136), e57506, doi:10.3791/57506 (2018).

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