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Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy
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Bioquímica
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JoVE Journal Bioquímica
Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy

Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy

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11:22 min

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June 27, 2018

DOI:

10.3791/57506-v

DOI:
10.3791/57506-v

•

11:22 min
June 27, 2018

•

1 Views
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1Laboratory of Molecular and Chemical Biology of Neurodegeneration,Ecole Polytechnique Fédérale de Lausanne (EPFL)

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Native Huntingtin Exon1SUMO Fusion StrategySDS-PAGE AnalysisIMAC PurificationHPLC PurificationDTTL-cysteineTrifluoroacetic AcidAggregation-prone ProteinsAmyloid-betaAlpha-synuclein

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Reif, A., Chiki, A., Ricci, J., Lashuel, H. A. Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy. J. Vis. Exp. (136), e57506, doi:10.3791/57506 (2018).

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