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Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format
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Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

6,743 Views

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08:35 min

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April 20, 2018

DOI:

10.3791/57305-v

DOI:
10.3791/57305-v

•

08:35 min
April 20, 2018

•

9 Views
, , ,
1Department of Neurosurgery,NorthShore University HealthSystem, 2Midwestern University/Chicago College of Osteopathic Medicine, 3Independent Contractor

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Here we present a method for a human in vitro model of stretch injury in a 96-well format on a timescale relevant to impact trauma. This includes methods for fabricating stretchable plates, quantifying the mechanical insult, culturing and injuring cells, imaging, and high content analysis to quantify injury.

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Phillips, J. K., Sherman, S. A., Oungoulian, S. R., Finan, J. D. Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format. J. Vis. Exp. (134), e57305, doi:10.3791/57305 (2018).

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