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Følgende endokardial væv bevægelser via celle Photoconversion i zebrafisk Embryo
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Developmental Biology
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JoVE Journal Developmental Biology
Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

Følgende endokardial væv bevægelser via celle Photoconversion i zebrafisk Embryo

DOI:
10.3791/57290-v

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09:38 min

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February 20, 2018

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, , , ,
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, 2UMR7104,Centre National de la Recherche Scientifique, 3U964,Institut National de la Santé et de la Recherche Médicale, 4Université de Strasbourg

Chapters

  • 00:04Title
  • 00:53Preparing Molds and Mounting Agarose and Embedding Embryos
  • 03:31Photoconversion
  • 05:12Unmounting Photoconverted Embryos
  • 06:20Imaging Photoconverted Cells at Later Embryonic Stages
  • 08:05Results: Photoconversion of Kaede Fluorescent Protein in Zebrafish Embryonic Endocardial Cells
  • 09:01Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver en metode til photoconversion af Kaede fluorescerende proteiner i endokardial celler levende zebrafisk embryon, der muliggør sporing af endokardial celler under atrioventrikulær canal og atrioventrikulær hjertet ventilen udvikling .

Tags

Cell PhotoconversionZebrafish EmbryoAtrioventricular CanalHeart Valve FormationCardiac DevelopmentMutantsTime-lapse MicroscopyAgaroseMounting MediumLow Melting Point AgaroseTricaineBDM

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